Small-angle X-ray scattering reveals differences between the quaternary structures of oxygenated and deoxygenated tarantula hemocyanin
Identifieur interne : 003E57 ( Main/Exploration ); précédent : 003E56; suivant : 003E58Small-angle X-ray scattering reveals differences between the quaternary structures of oxygenated and deoxygenated tarantula hemocyanin
Auteurs : Heinz Decker [Allemagne] ; Hermann Hartmann [Allemagne] ; Reinhard Sterner [Suisse] ; Erika Schwarz [Autriche] ; Ingrid Pilz [Autriche]Source :
- FEBS Letters [ 0014-5793 ] ; 1996.
English descriptors
- Teeft :
- Androctonus, Androctonus hemocyanin, Biol, Californicum, Conformational changes, Decker, Deoxygenated, Deoxygenated hemocyanin, Electron microscopy, Eurypelma, Hemocyanin, Hemocyanins, Hexamers, Limulus, Limulus hemocyanin, Limulus polyphemus, Oxygen binding, Oxygenated, Physiol, Polyphemus, Quaternary, Quaternary structure, Saxs measurements, Subunit, Tarantula, Tarantula hemocyanin.
Abstract
Abstract: Small-angle X-ray scattering (SAXS) curves have been recorded for the oxygenated and deoxygenated states of the 4 × 6-meric hemocyanin from the tarantula Eurypelma californicum. A comparison of the curves shows that the quaternary structures of the two states are different by three criteria, which all indicate that the hemocyanin is less compact in the oxygenated compared to the deoxygenated form: (a) The radius of gyration is 8.65 ± 0.05 nm for the deoxy- and 8.80 ± 0.05 nm for the oxy-form. (b) The maximum particle dimension amounts to 25.0 ± 0.5 nm for the deoxy- and to 27.0 ± 0.5 nm for the oxyform. (c) A dip in the intramolecular distance distribution function p(r) is more pronounced and shifted to larger distances in the oxy-form. The p(r) functions based on SAXS measurements were compared to p(r) functions deduced from published electron microscopical images of three different 4 × 6-meric hemocyanins from closely related species. The p(r) functions of SAXS and electron microscopy were similar in one case, whereas in the other two cases the distance between the two 12-meric half-molecules had to be changed by 1–1.5 nm to obtain good agreement. The differences between the p(r) functions of oxygenated and deoxygenated 4 × 6-meric tarantula hemocyanin are much larger than one would expect from a comparison of X-ray structures of the oxygenated and deoxygenated states of a closely related 6-meric hemocyanin. Thus, the conformational changes upon oxygenation occur at various levels of the quaternary structure, as postulated by hierarchical theories of allosteric interactions.
Url:
DOI: 10.1016/0014-5793(96)00887-3
Affiliations:
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structures of oxygenated and deoxygenated tarantula hemocyanin</title><author><name sortKey="Decker, Heinz" sort="Decker, Heinz" uniqKey="Decker H" first="Heinz" last="Decker">Heinz Decker</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Biophysics, University of Mainz, Welder-Weg 26, D-55099 Mainz</wicri:regionArea><wicri:noRegion>55099 Mainz</wicri:noRegion><wicri:noRegion>D-55099 Mainz</wicri:noRegion></affiliation></author><author><name sortKey="Hartmann, Hermann" sort="Hartmann, Hermann" uniqKey="Hartmann H" first="Hermann" last="Hartmann">Hermann Hartmann</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Biophysics, University of Mainz, Welder-Weg 26, D-55099 Mainz</wicri:regionArea><wicri:noRegion>55099 Mainz</wicri:noRegion><wicri:noRegion>D-55099 Mainz</wicri:noRegion></affiliation></author><author><name sortKey="Sterner, Reinhard" sort="Sterner, Reinhard" uniqKey="Sterner R" first="Reinhard" last="Sterner">Reinhard Sterner</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstr. 70, CH-4056 Basel</wicri:regionArea><wicri:noRegion>CH-4056 Basel</wicri:noRegion></affiliation></author><author><name sortKey="Schwarz, Erika" sort="Schwarz, Erika" uniqKey="Schwarz E" first="Erika" last="Schwarz">Erika Schwarz</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute for Physical Chemistry, University of Graz, Heinrichstr. 28, A-8010 Graz</wicri:regionArea><wicri:noRegion>A-8010 Graz</wicri:noRegion></affiliation></author><author><name sortKey="Pilz, Ingrid" sort="Pilz, Ingrid" uniqKey="Pilz I" first="Ingrid" last="Pilz">Ingrid Pilz</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute for Physical Chemistry, University of Graz, Heinrichstr. 28, A-8010 Graz</wicri:regionArea><wicri:noRegion>A-8010 Graz</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">FEBS Letters</title><title level="j" type="abbrev">FEBS</title><idno type="ISSN">0014-5793</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">393</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="226">226</biblScope><biblScope unit="page" to="230">230</biblScope></imprint><idno type="ISSN">0014-5793</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-5793</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Androctonus</term><term>Androctonus hemocyanin</term><term>Biol</term><term>Californicum</term><term>Conformational changes</term><term>Decker</term><term>Deoxygenated</term><term>Deoxygenated hemocyanin</term><term>Electron microscopy</term><term>Eurypelma</term><term>Hemocyanin</term><term>Hemocyanins</term><term>Hexamers</term><term>Limulus</term><term>Limulus hemocyanin</term><term>Limulus polyphemus</term><term>Oxygen binding</term><term>Oxygenated</term><term>Physiol</term><term>Polyphemus</term><term>Quaternary</term><term>Quaternary structure</term><term>Saxs measurements</term><term>Subunit</term><term>Tarantula</term><term>Tarantula hemocyanin</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Small-angle X-ray scattering (SAXS) curves have been recorded for the oxygenated and deoxygenated states of the 4 × 6-meric hemocyanin from the tarantula Eurypelma californicum. A comparison of the curves shows that the quaternary structures of the two states are different by three criteria, which all indicate that the hemocyanin is less compact in the oxygenated compared to the deoxygenated form: (a) The radius of gyration is 8.65 ± 0.05 nm for the deoxy- and 8.80 ± 0.05 nm for the oxy-form. (b) The maximum particle dimension amounts to 25.0 ± 0.5 nm for the deoxy- and to 27.0 ± 0.5 nm for the oxyform. (c) A dip in the intramolecular distance distribution function p(r) is more pronounced and shifted to larger distances in the oxy-form. The p(r) functions based on SAXS measurements were compared to p(r) functions deduced from published electron microscopical images of three different 4 × 6-meric hemocyanins from closely related species. The p(r) functions of SAXS and electron microscopy were similar in one case, whereas in the other two cases the distance between the two 12-meric half-molecules had to be changed by 1–1.5 nm to obtain good agreement. The differences between the p(r) functions of oxygenated and deoxygenated 4 × 6-meric tarantula hemocyanin are much larger than one would expect from a comparison of X-ray structures of the oxygenated and deoxygenated states of a closely related 6-meric hemocyanin. Thus, the conformational changes upon oxygenation occur at various levels of the quaternary structure, as postulated by hierarchical theories of allosteric interactions.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Autriche</li><li>Suisse</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Decker, Heinz" sort="Decker, Heinz" uniqKey="Decker H" first="Heinz" last="Decker">Heinz Decker</name></noRegion><name sortKey="Hartmann, Hermann" sort="Hartmann, Hermann" uniqKey="Hartmann H" first="Hermann" last="Hartmann">Hermann Hartmann</name></country><country name="Suisse"><noRegion><name sortKey="Sterner, Reinhard" sort="Sterner, Reinhard" uniqKey="Sterner R" first="Reinhard" last="Sterner">Reinhard Sterner</name></noRegion></country><country name="Autriche"><noRegion><name sortKey="Schwarz, Erika" sort="Schwarz, Erika" uniqKey="Schwarz E" first="Erika" last="Schwarz">Erika Schwarz</name></noRegion><name sortKey="Pilz, Ingrid" sort="Pilz, Ingrid" uniqKey="Pilz I" first="Ingrid" last="Pilz">Ingrid Pilz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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